目的 比较在线柱萃取高效液相色谱法(HPLC)与柱前衍生HPLC测定人血中丙戊酸(VPA)浓度的差异及相关性,为临床VPA血药浓度监测提供参考依据。方法 分别用2种方法对120份患者血样的VPA浓度进行检测,采用统计学方法对其检测结果的差异进行评价。结果 以柱前衍生HPLC测定结果(X)与在线柱萃取HPLC测定结果(Y)所作线性回归方程如下:Y=0.804 2X-2.611 3(r2=0.973 9),2种方法测定VPA血药浓度结果的差异有统计学意义(P<0.05)。结论 2种方法均可用于临床VPA的血药浓度监测,但对测得结果的差异应予以密切关注;相比柱前衍生HPLC,在线柱萃取HPLC操作简单且不易受其代谢物干扰,更适于临床VPA的血药浓度监测。
Abstract
OBJECTIVE To investigate the differences and correlation between the methods of on-line column extraction high performance liquid chromatography(HPLC) and pre-column derivatization HPLC for the determination of valproic acid(VPA) in plasma and provide reference for the clinical monitoring of VPA concentration. METHODS One hundred and twenty samples were detected by the two methods respectively, and the differences of the test results were evaluated by statistical methods. RESULTS The linear regression equation between the results determined by the pre-column derivatization HPLC method(X) and the on-line column HPLC method(Y) was as follows:Y=0.804 2X-2.611 3(r2=0.973 9). There was significant difference between the results determined by the two methods(P<0.05). CONCLUSION Both methods can be used to monitor the concentration of VPA in clinical practice. However, it is necessary to pay attention to the difference between the two methods. And the on-line column HPLC method is more suitable for monitoring the concentration of VPA in clinical practice because it is simple to operate and has less interference from the metabolites.
关键词
丙戊酸 /
在线柱萃取高效液相色谱法 /
柱前衍生高效液相色谱法 /
治疗药物监测
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Key words
valproic acid /
on-line column extraction HPLC /
pre-column derivatization HPLC /
therapeutic drug monitoring
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中图分类号：
R965
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参考文献
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